Investigation of singlet oxygen generation in Vit C-Cu2+-LDL system by chemiluminescence method

Juan Wang; Da Xing; Shici Tan; Yonghong Tang; Yonghong He

doi:10.1117/12.462544

12 April 2002 Investigation of singlet oxygen generation in Vit C-Cu²⁺-LDL system by chemiluminescence method

Juan Wang, Da Xing, Shici Tan, Yonghong Tang, Yonghong He

Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002) https://doi.org/10.1117/12.462544
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China

Abstract

In this study, by chemiluminescence method using a Cypridina luciferin analog, 2-methyl-6-(p-methoxyphenyl)-3,7- dihydroimidazo[1,2-a]pyrazin-3-one (MCLA), as a selective and sensitive chemiluminescence probe, singlet oxygen (¹O₂) formation was observed in the vit C- LDL-Cu²⁺ reaction system. Another experimental evidence for the generation of ¹O₂ was the quenching effect of sodium azide (NaN₃) on vit C-induced chemiluminescence in the reaction mixture of LDL- Cu²⁺-MCLA. Analysis based on the experimental results indicated the plausible reaction mechanism is that vit C converts Cu²⁺ to its reduced state and vit C becomes vit C radical itself, thereby stimulating the formation of peroxyl radicals, and bimolecular reaction of peroxyl radicals results in ¹O₂ production in the above systems.

Citation Download Citation

Juan Wang, Da Xing, Shici Tan, Yonghong Tang, and Yonghong He "Investigation of singlet oxygen generation in Vit C-Cu²⁺-LDL system by chemiluminescence method", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); https://doi.org/10.1117/12.462544

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