Application of multiphoton steady state and lifetime imaging to mapping of tumor vascular architecture in vivo

Simon Ameer-Beg; Paul R. Barber; R. J. Hodgkiss; R. J. Locke; Robert G. Newman; Gillian M. Tozer; Borivoj Vojnovic; J. Wilson

doi:10.1117/12.470681

17 June 2002 Application of multiphoton steady state and lifetime imaging to mapping of tumor vascular architecture in vivo

Simon Ameer-Beg, Paul R. Barber, R. J. Hodgkiss, R. J. Locke, Robert G. Newman, Gillian M. Tozer, Borivoj Vojnovic, J. Wilson

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Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470681
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Recent interest in vascular targeting and anti-angiogenic drug treatments for cancer has stimulated fundamental research regarding the modes of action of these drugs as well as studies of the development and re-modeling of the vascular network following treatment. Multiphoton fluorescence microscopy is employed for in vivo mapping of three-dimensional blood vessel distribution in tumors grown in rodent dorsal skin-flap window chamber preparations. Accurate visualization of the vasculature in three-dimensions allows us to perform dynamic experiments in thick biological specimens in vivo. Examples of in vivo imaging of tumor vasculature are given and compared to normal tissue vasculature. The dynamic responses of blood vessels to treatment with the vascular targeting drug combretastatin A4-P are presented and discussed. The implementation of time-domain imaging by reversed stop-start time-correlated single photon counting (RSS-TCSPC) is discussed as a method for feature extraction in the presence of exogenous and endogenous fluorophores. In particular, the segmentation of the vascular network is demonstrated. Additional contrast, indicative of probe environmental factors, may also be realized. We present examples of in vivo lifetime imaging as a method to elucidate the physiological processes of the tumor microenvironment.

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Simon Ameer-Beg, Paul R. Barber, R. J. Hodgkiss, R. J. Locke, Robert G. Newman, Gillian M. Tozer, Borivoj Vojnovic, and J. Wilson "Application of multiphoton steady state and lifetime imaging to mapping of tumor vascular architecture in vivo", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470681

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