Soft lithography and surface chemistry: enabling tools for new bioassays

Bernardo D. Aumond; Oliver Schueller; David Duffy; Emanuele Ostuni; Enoch Kim; Aaron Raphel; Christian Hodneland; Stewart Campbell; Gregory Kirk

doi:10.1117/12.491185

21 June 2002 Soft lithography and surface chemistry: enabling tools for new bioassays

Bernardo D. Aumond, Oliver Schueller, David Duffy, Emanuele Ostuni, Enoch Kim, Aaron Raphel, Christian Hodneland, Stewart Campbell, Gregory Kirk

Author Affiliations +

Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002) https://doi.org/10.1117/12.491185
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The process of drug discovery can be accelerated by increasing the information content of bioassays and by employing assay platforms that are amenable to high throughput screening techniques. In this paper, we demonstrate how the combination of soft lithography with controlled surface chemistry achieves these goals in a wide spectrum of bioassays. A number of soft lithographic methods can be used to generate micro-structures for the purposes of increasing assay density, diversity of test conditions and improving assay detection qualities. In addition, soft lithography, combined with specific surface chemistry modification procedures and protein engineering, may be used to control the localized molecular and biological properties of substrates, thereby enabling the development of new types of bioassays. The developed methodologies are simple, easily implemented, and lend themselves well to automation. Experimental results and prototypes are presented to illustrate the capabilities of these new techniques. For example, soft lithography and surface chemistry are employed for chemically patterning substrates, stenciling biological entities onto substrates and confining solutions. As a result, information-rich, highdensity bioassays can be obtained where biological targets, surface properties and medium solutions are carefully determined and controlled.

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Bernardo D. Aumond, Oliver Schueller, David Duffy, Emanuele Ostuni, Enoch Kim, Aaron Raphel, Christian Hodneland, Stewart Campbell, and Gregory Kirk "Soft lithography and surface chemistry: enabling tools for new bioassays", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); https://doi.org/10.1117/12.491185

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KEYWORDS

Lithography

Optical lithography

Chemistry

Chemical elements

Proteins

Self-assembled monolayers

Printing

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