Point spread function model for microscopic image deconvolution in digital holographic microscopy

Anca Marian; Etienne Cuche; Christian D. Depeursinge

doi:10.1117/12.500614

14 October 2003 Point spread function model for microscopic image deconvolution in digital holographic microscopy

Anca Marian, Etienne Cuche, Christian D. Depeursinge

Proceedings Volume 5143, Novel Optical Instrumentation for Biomedical Applications; (2003) https://doi.org/10.1117/12.500614
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

A simple model is proposed in order to evaluate the complex amplitude point spread function (intensity and phase) of a microscope objective. The model is based on the Fresnel diffraction theory and takes also into account the possible optical aberrations. Experimental evaluation of this amplitude point spread function has been carried out by using a holographic set-up and 60 nanometers gold spheres as punctual objects. The measured values are then compared with the theoretical predicted model.

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Anca Marian, Etienne Cuche, and Christian D. Depeursinge "Point spread function model for microscopic image deconvolution in digital holographic microscopy", Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); https://doi.org/10.1117/12.500614

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