Short-coherence digital holography for the investigation of 3D microscopic samples

Lluis Martinez-Leon; Giancarlo Pedrini; Wolfgang Osten

doi:10.1117/12.544107

10 September 2004 Short-coherence digital holography for the investigation of 3D microscopic samples

Lluis Martinez-Leon, Giancarlo Pedrini, Wolfgang Osten

Proceedings Volume 5457, Optical Metrology in Production Engineering; (2004) https://doi.org/10.1117/12.544107
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

An optical system based on short-coherence digital holography suitable for three-dimensional microscopic investigations is described. An in-line arrangement is used, in which the light source is a short-coherence laser and the holograms are recorded on a CCD sensor. The interference (hologram) occurs only when the path lengths of the reference and the object beam are matched within the coherence length of the laser. The phase of the object wave front in the plane of the CCD detector is determined by phase shifting. The image of the part of the sample that matches the reference beam is reconstructed by numerical evaluation of the hologram. The three-dimensional scanning of the sample is easily obtained by shifting the position of a mirror in the reference arm of the interferometer. Reconstruction of different layers of the sample is possible up to a depth of several hundred micrometers. However, in tissues and other biological samples, while the surface of the sample is properly reconstructed, the multiple scattering and the inhomogeneties in the refractive index reduce the imaging quality of the deepest layers. The possibility to correct numerically this sample-induced aberrations has been studied. Simulations and experimental results are presented.

Citation Download Citation

Lluis Martinez-Leon, Giancarlo Pedrini, and Wolfgang Osten "Short-coherence digital holography for the investigation of 3D microscopic samples", Proc. SPIE 5457, Optical Metrology in Production Engineering, (10 September 2004); https://doi.org/10.1117/12.544107

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

3D image reconstruction

Holograms

Digital holography

Charge-coupled devices

Holography

Tissues

Sensors

Show All Keywords

Keywords/Phrases

Search In:

Publication Years