Autofocus algorithm using one-dimensional Fourier transform and Pearson correlation

A. Bueno Mario; Josue Alvarez-Borrego; L. Acho

doi:10.1117/12.592180

21 October 2004 Autofocus algorithm using one-dimensional Fourier transform and Pearson correlation

A. Bueno Mario, Josue Alvarez-Borrego, L. Acho

Proceedings Volume 5622, 5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications; (2004) https://doi.org/10.1117/12.592180
Event: 5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications, 2004, Porlamar, Venezuela

Abstract

A new autofocus algorithm based on one-dimensional Fourier transform and Pearson correlation for Z automatized microscope is proposed. Our goal is to determine in fast response time and accuracy, the best focused plane through an algorithm. We capture in bright and dark field several images set at different Z distances from biological organism sample. The algorithm uses the one-dimensional Fourier transform to obtain the image frequency content of a vectors pattern previously defined comparing the Pearson correlation of these frequency vectors versus the reference image frequency vector, the most out of focus image, we find the best focusing. Experimental results showed the algorithm has fast response time and accuracy in getting the best focus plane from captured images. In conclusions, the algorithm can be implemented in real time systems due fast response time, accuracy and robustness. The algorithm can be used to get focused images in bright and dark field and it can be extended to include fusion techniques to construct multifocus final images beyond of this paper.

Citation Download Citation

A. Bueno Mario, Josue Alvarez-Borrego, and L. Acho "Autofocus algorithm using one-dimensional Fourier transform and Pearson correlation", Proc. SPIE 5622, 5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications, (21 October 2004); https://doi.org/10.1117/12.592180

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