Sensitive time-resolved fluorometer

Zhen Tian; Zhouyi Guo

doi:10.1117/12.574539

10 February 2005 Sensitive time-resolved fluorometer

Zhen Tian, Zhouyi Guo

Proceedings Volume 5638, Optical Design and Testing II; (2005) https://doi.org/10.1117/12.574539
Event: Photonics Asia, 2004, Beijing, China

Abstract

With the use of lanthanide trivalence ion and its chelates for marking substance, Time-resolved Fluorescence Immunoassay (TRFIA) is superior to RIA in sensitivity, speciality, stability. And it has become one of the hotspots in the field of labeled immunoassay application. In this paper, a sensitive time-resolved fluorescence immunoassay instrument made by us was presented. In the device, a pulsed Xenon lamp of flash frequency 1kHz was adopted as exciting light, and the peculiarities of long lifetime and big Stokes about lanthanide trivalence ion and its chelates was utilized for the application of time-resolved and spectra-resolved technique. Thus the influence of scattering light and short-lifetime fluorescence was removed. By testing, the sensitivity is 10^-12mol/L (when Eu³⁺ was used for marking substance), examination repeat is CV less than or equal to 5%, examination linearity is from 10^-8mol/L to 10^-12mol/L, correlation coefficient r=99.9%(p=0.0001). In a word, the instrument is advanced for ultrasensitive detection of antigen and antibody, and many other aspects.

Citation Download Citation

Zhen Tian and Zhouyi Guo "Sensitive time-resolved fluorometer", Proc. SPIE 5638, Optical Design and Testing II, (10 February 2005); https://doi.org/10.1117/12.574539

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