Refractive index changes during germination of bacillus subtilis spores

Alvin Katz; Alexandra Alimova; Min Xu; Elizabeth Rudolph; Paul Gottlieb; J. C. Steiner; Robert R. Alfano

doi:10.1117/12.588590

1 April 2005 Refractive index changes during germination of bacillus subtilis spores

Alvin Katz, Alexandra Alimova, Min Xu, Elizabeth Rudolph, Paul Gottlieb, J. C. Steiner, Robert R. Alfano

Proceedings Volume 5692, Advanced Biomedical and Clinical Diagnostic Systems III; (2005) https://doi.org/10.1117/12.588590
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Light transmission measurements in the wavelength range of 400 to 1000 nm were performed on Bacillus subtilis spores at periodic time intervals after heat-shock induced activation. The Gaussian ray approximation, using a concentric sphere model for the spore coat and spore core, was used to calculate the scattering cross-section of the spores. Analysis of transmission spectra determined that the refractive index of the spore core was 1.515. In the three hours following heat shock, the core refractive index decreased to 1.39, and subsequently remained constant. During the measurements, the spore radius increased from 0.38 microns to 0.6 microns. The results were confirmed by phase contrast microscopy.

Citation Download Citation

Alvin Katz, Alexandra Alimova, Min Xu, Elizabeth Rudolph, Paul Gottlieb, J. C. Steiner, and Robert R. Alfano "Refractive index changes during germination of bacillus subtilis spores", Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); https://doi.org/10.1117/12.588590

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