Two-dimensional micro array fluorescence fingerprinting with a computer screen photo-assisted technique

Jimmy W. P. Bakker; Daniel Filippini; Ingemar Lundstroem

doi:10.1117/12.589586

23 March 2005 Two-dimensional micro array fluorescence fingerprinting with a computer screen photo-assisted technique

Jimmy W. P. Bakker, Daniel Filippini, Ingemar Lundstroem

Proceedings Volume 5694, Spectral Imaging: Instrumentation, Applications, and Analysis III; (2005) https://doi.org/10.1117/12.589586
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Detection and classification of fluorescent dyes are demonstrated using a computer screen photo-assisted technique (CSPT). This technique has previously been demonstrated for analyzing fluorescence from 96 wells microtiterplates (200 μl per well) and from a single cuvette with some optics to enhance sensitivity. In this work a custom designed array of wells with a volume of approximately 1 mu;l is used. In order to measure such small volumes without saturating the detector, the transmitted light is masked by placing the sample between two crossed polarizers. This arrangement blocks nearly all the transmitted light, while the emitted light, which is nearly unpolarized, can still be detected. The lowest amount (concentration x volume) of analyte detectable in this setup is about 40 times smaller than in the previous setups.

Citation Download Citation

Jimmy W. P. Bakker, Daniel Filippini, and Ingemar Lundstroem "Two-dimensional micro array fluorescence fingerprinting with a computer screen photo-assisted technique", Proc. SPIE 5694, Spectral Imaging: Instrumentation, Applications, and Analysis III, (23 March 2005); https://doi.org/10.1117/12.589586

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