Transmission holographic microscope: image characteristics

Radim Chmelík; Pavel Kolman; Ludék Lovicar; Filip Suchomel

doi:10.1117/12.638945

6 December 2006 Transmission holographic microscope: image characteristics

Radim Chmelík, Pavel Kolman, Ludék Lovicar, Filip Suchomel

Proceedings Volume 5945, 14th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics; 59450V (2006) https://doi.org/10.1117/12.638945
Event: 14th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 2005, Nitra, Slovakia

Abstract

The intensity of the reconstructed image in the transmission holographic microscope is depth discriminated as it is in a transmission confocal microscope. The effect is the consequence of the limited coherence of the illumination - hence no scanning system is needed. As the technique is based on the incoherent holography, the phase image component may be reconstructed in addition to the intensity one. The overall imaging process is coherent. Its three-dimensional coherent transfer function is derived using the first Born approximation of the scattering theory. In order to understand clearly the imaging process of the microscope, two-dimensional imaging characteristics are derived in this paper in addition to the three-dimensional one, and images of a rectilinear slit as a model two-dimensional structure are calculated for various amounts of defocus. Theoretical axial distributions of the intensity integral are compared with the experimental ones.

Citation Download Citation

Radim Chmelík, Pavel Kolman, Ludék Lovicar, and Filip Suchomel "Transmission holographic microscope: image characteristics", Proc. SPIE 5945, 14th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 59450V (6 December 2006); https://doi.org/10.1117/12.638945

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Microscopes

Holography

3D image reconstruction

Image processing

Holograms

Confocal microscopy

Diffusers

Show All Keywords

Keywords/Phrases

Search In:

Publication Years