Changes in the aqueous phase of cell suspensions and protein solutions induced by a photoexitation of the molecular oxygen (to mechanisms of biological action of low level laser radiation)

S. D. Zakharov; A. V. Ivanov

doi:10.1117/12.640184

7 December 2006 Changes in the aqueous phase of cell suspensions and protein solutions induced by a photoexitation of the molecular oxygen (to mechanisms of biological action of low level laser radiation )

S. D. Zakharov, A. V. Ivanov

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Proceedings Volume 5973, Current Research on Laser Use in Oncology: 2000-2004; 59730V (2006) https://doi.org/10.1117/12.640184
Event: Current Research on Laser Use in Oncology: 2000-2004, 2004, Moscow, Russian Federation

Abstract

Temperature-dependent transient processes initiated by a direct photogeneration of singlet oxygen in suspensions of human erythrocytes and solutions of serum albumin are studied. The processes appear as anomalous jumps in the temperature dependences of the deformability coefficient of erythrocytes and the refractive index of the extracellular medium and protein solution. In the temperature regions of anomalous jumps, cells and proteins transfer to a metastable state of a lower activity, but they can be isothermally photoreactivated. Simultaneously, a reversible rearrangement of the aqueous phase occurs near the cell and protein surfaces, accompanied by the formation of an extended corona (hydrogel). The transient processes are interpreted as phase transitions in the membrane of erythrocytes and conformation transitions in proteins. The interaction between erythrocytes and albumin via hydrogel is discovered (hydro-conformational interaction).

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S. D. Zakharov and A. V. Ivanov "Changes in the aqueous phase of cell suspensions and protein solutions induced by a photoexitation of the molecular oxygen (to mechanisms of biological action of low level laser radiation )", Proc. SPIE 5973, Current Research on Laser Use in Oncology: 2000-2004, 59730V (7 December 2006); https://doi.org/10.1117/12.640184

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KEYWORDS

Proteins

Oxygen

Refraction

Refractive index

Prisms

Molecules

Blood

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