Portable real-time fluorescence cytometry of microscale cell culture analog devices

Donghyun Kim; Daniel A. Tatosian; Michael L. Shuler

doi:10.1117/12.644521

22 February 2006 Portable real-time fluorescence cytometry of microscale cell culture analog devices

Donghyun Kim, Daniel A. Tatosian, Michael L. Shuler

Proceedings Volume 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV; 60880X (2006) https://doi.org/10.1117/12.644521
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.

Citation Download Citation

Donghyun Kim, Daniel A. Tatosian, and Michael L. Shuler "Portable real-time fluorescence cytometry of microscale cell culture analog devices", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 60880X (22 February 2006); https://doi.org/10.1117/12.644521

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KEYWORDS

Luminescence

Green fluorescent protein

Light emitting diodes

Light sources

Fluorescence spectroscopy

Microscopes

Analog electronics

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