Digital holographic microscopy: a new optical imaging technique to investigate cellular dynamics

Pierre Marquet; Benjamin Rappaz; Tristan Colomb; Florian Charrière; Jonas Kühn; Yves Emery; Etienne Cuche; Christian Depeursinge; Pierre Magistretti

doi:10.1117/12.662988

14 April 2006 Digital holographic microscopy: a new optical imaging technique to investigate cellular dynamics

Pierre Marquet, Benjamin Rappaz, Tristan Colomb, Florian Charrière, Jonas Kühn, Yves Emery, Etienne Cuche, Christian Depeursinge, Pierre Magistretti

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Proceedings Volume 6191, Biophotonics and New Therapy Frontiers; 61910U (2006) https://doi.org/10.1117/12.662988
Event: SPIE Photonics Europe, 2006, Strasbourg, France

Abstract

We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to λ/1800, and the low acquisition time (down to 20 μs) enables to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the intracellular refractive index and the cellular thickness (morphometry) from the measured phase shift is presented.

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Pierre Marquet, Benjamin Rappaz, Tristan Colomb, Florian Charrière, Jonas Kühn, Yves Emery, Etienne Cuche, Christian Depeursinge, and Pierre Magistretti "Digital holographic microscopy: a new optical imaging technique to investigate cellular dynamics", Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910U (14 April 2006); https://doi.org/10.1117/12.662988

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