Application of capillary electrophoresis to the development and evaluation of aptamer affinity probes

Letha J. Sooter; Sun McMasters; Dimitra N. Stratis-Cullum

doi:10.1117/12.731962

5 October 2007 Application of capillary electrophoresis to the development and evaluation of aptamer affinity probes

Letha J. Sooter, Sun McMasters, Dimitra N. Stratis-Cullum

Proceedings Volume 6759, Smart Biomedical and Physiological Sensor Technology V; 67590T (2007) https://doi.org/10.1117/12.731962
Event: Optics East, 2007, Boston, MA, United States

Abstract

Nucleic acid aptamers can exhibit high binding affinities for a wide variety of targets and have received much attention as molecular recognition elements for enhanced biosensor performance. These aptamers recognize target molecules through a combination of conformational dependent non-covalent interactions in aqueous media which can be investigated using capillary electrophoresis-based methods. In this paper we report on the results of our studies of the relative binding affinity of Campylobacter jejuni aptamers using a capillary electrophoretic immunoassay. Our results show preferential binding to C. jejuni over other common food pathogen bacteria. Capillary electrophoresis can also be used to develop new aptamer recognition elements using an in vitro selection process known as systematic evolution of ligand by exponential enrichment (SELEX). Recently, this process has been adapted to use capillary electrophoresis in an attempt to shorten the overall selection process. This smart selection of nucleic acid aptamers from a large diversity of a combinatorial DNA library is under optimization for the development of aptamers which bind to Army-relevant targets. This paper will include a discussion of the establishment of CE-SELEX methods for the future development of smart aptamer probes.

Citation Download Citation

Letha J. Sooter, Sun McMasters, and Dimitra N. Stratis-Cullum "Application of capillary electrophoresis to the development and evaluation of aptamer affinity probes", Proc. SPIE 6759, Smart Biomedical and Physiological Sensor Technology V, 67590T (5 October 2007); https://doi.org/10.1117/12.731962

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available