A microflow cytometer on a chip

Joel P. Golden; Jason Kim; George P. Anderson; Nastaran Hashemi; Peter J. Howell; Frances S. Ligler

doi:10.1117/12.842623

25 February 2010 A microflow cytometer on a chip

Joel P. Golden, Jason Kim, George P. Anderson, Nastaran Hashemi, Peter J. Howell, Frances S. Ligler

Proceedings Volume 7553, Frontiers in Pathogen Detection: From Nanosensors to Systems; 755308 (2010) https://doi.org/10.1117/12.842623
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A rapid, automated, multi-analyte Microflow Cytometer is being developed as a portable, field-deployable sensor for onsite diagnosis of biothreat agent exposure and environmental monitoring. The technology relies on a unique method for ensheathing a sample stream in continuous flow past an interrogation region where optical fibers provide excitation and collect emission. This approach efficiently focuses particles in the interrogation region of the fluidic channel, avoids clogging and provides for subsequent separation of the core and sheath fluids in order to capture the target for confirmatory assays and recycling of the sheath fluid. Fluorescently coded microspheres provide the capability for highly multiplexed assays. Optical analysis at four different wavelengths identified six sets of the coded microspheres recognizing Escherichia coli, Listeria, and Salmonella as well as cholera toxin, staphylococcal enterotoxin B (SEB), and ricin, and assay results were compared with those of a commercial Luminex analysis system.

Citation Download Citation

Joel P. Golden, Jason Kim, George P. Anderson, Nastaran Hashemi, Peter J. Howell, and Frances S. Ligler "A microflow cytometer on a chip", Proc. SPIE 7553, Frontiers in Pathogen Detection: From Nanosensors to Systems, 755308 (25 February 2010); https://doi.org/10.1117/12.842623

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available