High-efficiency single molecule fluorescence detection and correlation spectroscopy with dielectric microspheres

Heykel Aouani; Nadia Djaker; Jérôme Wenger; Hervé Rigneault

doi:10.1117/12.840041

25 February 2010 High-efficiency single molecule fluorescence detection and correlation spectroscopy with dielectric microspheres

Heykel Aouani, Nadia Djaker, Jérôme Wenger, Hervé Rigneault

Author Affiliations +

Proceedings Volume 7571, Single Molecule Spectroscopy and Imaging III; 75710A (2010) https://doi.org/10.1117/12.840041
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Microspheres made of high refractive index melamine resin are shown to enhance the fluorescence from single molecules in solution by seven-fold, and simultaneously reduce the observation volume by thirteen-fold, as compared to state-of-the-art confocal microscopy. This fluorescence enhancement is demonstrated to dramatically increase the signal-to-noise ratio in fluorescence correlation spectroscopy and reduce the experiment integration time by fifty-fold. We also provide the first description of dual-color fluorescence cross-correlation spectroscopy (FCCS) enhanced by a dielectric microsphere, and report comparable enhancement factors as for the single color case.

Citation Download Citation

Heykel Aouani, Nadia Djaker, Jérôme Wenger, and Hervé Rigneault "High-efficiency single molecule fluorescence detection and correlation spectroscopy with dielectric microspheres", Proc. SPIE 7571, Single Molecule Spectroscopy and Imaging III, 75710A (25 February 2010); https://doi.org/10.1117/12.840041

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