Second harmonic phase microscopy of collagen fibers

Etienne Shaffer; Pierre Marquet; Christian Depeursinge

doi:10.1117/12.874538

11 February 2011 Second harmonic phase microscopy of collagen fibers

Etienne Shaffer, Pierre Marquet, Christian Depeursinge

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79030G (2011) https://doi.org/10.1117/12.874538
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional information, inaccessible to incoherent imaging schemes. Last year, we have reported how the SHG phase makes possible real-time nanometric 3D-tracking of SHG emitters, such as nanoparticles (Shaffer et al., Opt. Express 18, p.17392-17403, 2010). Here, we investigate the phase of second harmonic generated by a label-free biological specimen-more precisely collagen fibers forming the connective tissue of a mouse dermis- and discuss of its interpretation. Notably, we show how the SHG phase, qualitatively acting as an indicator of phase-matching conditions, tends to indicate that second harmonic generation, in collagen, is dominated by coherent SHG scattering.

Citation Download Citation

Etienne Shaffer, Pierre Marquet, and Christian Depeursinge "Second harmonic phase microscopy of collagen fibers", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030G (11 February 2011); https://doi.org/10.1117/12.874538

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