Optically sectioned imaging by oblique plane microscopy

Sunil Kumar; Ziduo Lin; Alex R. Lyon; Ken T. MacLeod; Chris Dunsby

doi:10.1117/12.873471

28 February 2011 Optically sectioned imaging by oblique plane microscopy

Sunil Kumar, Ziduo Lin, Alex R. Lyon, Ken T. MacLeod, Chris Dunsby

Proceedings Volume 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII; 79040X (2011) https://doi.org/10.1117/12.873471
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Oblique Plane Microscopy (OPM) is a light sheet microscopy technique that combines oblique illumination with correction optics that tilt the focal plane of the collection system. OPM can be used to image conventionally mounted specimens on coverslips or tissue culture dishes and has low out-of-plane photobleaching and phototoxicity. No moving parts are required to achieve an optically sectioned image and so high speed optically sectioned imaging is possible. The first OPM results obtained using a high NA water immersion lens on a commercially available inverted microscope frame are presented, together with a measurement of the achievable optical resolution.

Citation Download Citation

Sunil Kumar, Ziduo Lin, Alex R. Lyon, Ken T. MacLeod, and Chris Dunsby "Optically sectioned imaging by oblique plane microscopy", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040X (28 February 2011); https://doi.org/10.1117/12.873471

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