Photo-cycle dynamics of LOV1-His domain of phototropin from Chlamydomonas reinhardtii with roseoflavin monophosphate cofactor

A. Tyagi; A. Penzkofer; T. Mathes; P. Hegemann

doi:10.1117/12.879134

11 January 2011 Photo-cycle dynamics of LOV1-His domain of phototropin from Chlamydomonas reinhardtii with roseoflavin monophosphate cofactor

A. Tyagi, A. Penzkofer, T. Mathes, P. Hegemann

Author Affiliations +

Proceedings Volume 7993, ICONO 2010: International Conference on Coherent and Nonlinear Optics; 79931E (2011) https://doi.org/10.1117/12.879134
Event: International Conference on Coherent and Nonlinear Optics (ICONO 2010) and International Conference on Lasers, Applications and Technologies (LAT 2010), 2010, Kazan, Russian Federation

Abstract

The wild-type phototropin protein phot from the green alga Chlamydomonas reinhardtii consists of two N-terminal LOV domains LOV1 and LOV2 with flavin mononucleotide (FMN) cofactor and a C-terminal serine-threonine kinase domain. It controls multiple steps in the sexual lifecycle of the alga. Here the LOV1-His domain of phot with modified cofactor is studied. FMN is replaced by roseoflavin monophosphate (8-dimethylamino-8-demethyl-FMN, RoFMN). The modified LOV1 domain is called RoLOV1. The photo-dynamics consequences of the cofactor change are studied. The absorption, emission, and photo-cyclic behaviour of LOV1-His and RoLOV1-His are compared. A spectroscopic characterisation of the cofactors FMN and RoFMN (roseoflavin) is given.

Citation Download Citation

A. Tyagi, A. Penzkofer, T. Mathes, and P. Hegemann "Photo-cycle dynamics of LOV1-His domain of phototropin from Chlamydomonas reinhardtii with roseoflavin monophosphate cofactor", Proc. SPIE 7993, ICONO 2010: International Conference on Coherent and Nonlinear Optics, 79931E (11 January 2011); https://doi.org/10.1117/12.879134

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