Amplification-free point of care immunosensor for detecting type V collagen at a concentration level of ng/ml

Pei-Yu Chung; Evelyn R. Bracho-Sanchez; Peng Jiang; JeanClare Seagrave; Matthew R. Duncan; Gary R. Grotendorst; Gregory Schultz; Christopher Batich

doi:10.1117/12.887147

16 May 2011 Amplification-free point of care immunosensor for detecting type V collagen at a concentration level of ng/ml

Pei-Yu Chung, Evelyn R. Bracho-Sanchez, Peng Jiang, JeanClare Seagrave, Matthew R. Duncan, Gary R. Grotendorst, Gregory Schultz, Christopher Batich

Author Affiliations +

Proceedings Volume 8029, Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring; and Biometric Technology for Human Identification VIII; 80291C (2011) https://doi.org/10.1117/12.887147
Event: SPIE Defense, Security, and Sensing, 2011, Orlando, Florida, United States

Abstract

Point-of-care testing (POCT) is applicable in the immediate vicinity of the patient, where timely diagnosis or prognostic information could help doctors decide the following treatment. Among types of developed POCT, gold nanoparticle based lateral flow strip technology provides advantages such as simple operation, cost-effectiveness, and a user-friendly platform. Therefore, this type of POCT is most likely to be used in battlefields and developing countries. However, conventional lateral flow strips suffer from low detection limits. Although enzyme-linked amplification was demonstrated to improve the detection limit and sensitivity by stronger visible lines or by permitting electrochemical analytical instrumentation, the enzyme labels have potential to cause interference with other enzymes in our body fluids. To eliminate this limitation, we developed an amplification-free gold nanoparticle-based immunosensor applied for detecting collagen type V, which is produced or released abnormally during rejection of lung transplants and sulfur mustard exposure. By using suitable blocking protein to stabilize gold nanoparticles as the reporter probe, a low detection limit of ng/ml was achieved. This strategy is a promising platform for clinical POCT, with potential applications in military or disaster response.

Citation Download Citation

Pei-Yu Chung, Evelyn R. Bracho-Sanchez, Peng Jiang, JeanClare Seagrave, Matthew R. Duncan, Gary R. Grotendorst, Gregory Schultz, and Christopher Batich "Amplification-free point of care immunosensor for detecting type V collagen at a concentration level of ng/ml", Proc. SPIE 8029, Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring; and Biometric Technology for Human Identification VIII, 80291C (16 May 2011); https://doi.org/10.1117/12.887147

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available