Spectroscopic-tomography of biological membrane with high-spatial resolution by the imaging-type 2D Fourier spectroscopy

Asuka Inui; Ryosuke Tsutsumi; Wei Qi; Takashi Takuma; Ichirou Ishimaru

doi:10.1117/12.890032

10 June 2011 Spectroscopic-tomography of biological membrane with high-spatial resolution by the imaging-type 2D Fourier spectroscopy

Asuka Inui, Ryosuke Tsutsumi, Wei Qi, Takashi Takuma, Ichirou Ishimaru

Author Affiliations +

Proceedings Volume 8087, Clinical and Biomedical Spectroscopy and Imaging II; 80871W (2011) https://doi.org/10.1117/12.890032
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

We proposed the imaging-type 2-dimensional Fourier spectroscopy that is the phase-shift interferometry between the objective lights. The proposed method can measure the 2D spectral image at the limited depth. Because of the imaging optical system, the 2D spectral images can be measured in high spatial resolution. And in the depth direction, we can get the spectral distribution only in the focal plane. In this report, we mention about the principle of the proposed wide field imaging-type 2D Fourier spectroscopy. And, we obtained the spectroscopic tomography of biological tissue of mouse's ear. In the visible region, we confirmed the difference of spectral characteristics between blood vessel region and other region. In the near infrared region (λ=900nm~1700nm), we can obtain the high-contrast blood vessel image of mouse's ear in the deeper part by InGaAs camera. Furthermore, in the middle infrared region(λ=8μ~14μm), we have successfully measured the radiation spectroscopic-imaging with wild field of view by the infrared module, such as the house plants. Additionally, we propose correction geometrical model that can convert the mechanical phase-shift value into the substantial phase difference in each oblique optical axes. We successfully verified the effectiveness of the proposed correction geometrical model and can reduce the spectral error into the error range into ±3nm using the He-Ne laser whose wavelength 632.8nm.

Citation Download Citation

Asuka Inui, Ryosuke Tsutsumi, Wei Qi, Takashi Takuma, and Ichirou Ishimaru "Spectroscopic-tomography of biological membrane with high-spatial resolution by the imaging-type 2D Fourier spectroscopy", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80871W (10 June 2011); https://doi.org/10.1117/12.890032

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