Light scattering spectra of sulfur-reducing bacteria Desulfuromonas acetoxidans under the influence of ions Fe metals

Oleksandr I. Bilyy; Oresta M. Vasyliv; Svitlana O. Hnatush

doi:10.1117/12.916150

22 November 2011 Light scattering spectra of sulfur-reducing bacteria Desulfuromonas acetoxidans under the influence of ions Fe metals

Oleksandr I. Bilyy, Oresta M. Vasyliv, Svitlana O. Hnatush

Author Affiliations +

Proceedings Volume 8338, Tenth International Conference on Correlation Optics; 83381I (2011) https://doi.org/10.1117/12.916150
Event: Correlation Optics 2011, 2011, Chernivsti, Ukraine

Abstract

Desulfuromonas acetoxidans are uncoloured gram-negative sulfur bacteria that inhabit sulfur containing aquatic environments. These are gram-negative obligatory anaerobes that have an ability to reduce S⁰ to H₂S and Fe³⁺ to Fe²⁺ in the processes of dissimilation Sulfur- and Fe (III)-reduction. Existence of Sulfur and Ferric ions in the anaerobic sediments causes binding of hydrogen sulfide which diffuses from the zone of sulfur reducing. As a result hydrogen sulfide is detoxificated. The ability of these bacteria to use Sulfur or Ferric ions as acceptors of electrons during organic carbon oxidation causes their special adaptation to the changes of surrounding environment. Interaction between bacterial Desulfuromonas acetoxidans cells and different concentrations of ferrous Fe²⁺ and ferric Fe³⁺ ions possibly could cause the changes of cells' light scattering characteristics. The changes of cells relative content and their size distribution during five days of cultivation under the influence of FeSO4 and FeCl₃×6H₂O in concentration from 0.01 to 10 mM was investigated by the new method of measurement. It includes sounding of flow suspended bacterial cells by monochromatic coherent light, registration of signals of co-operation of sounding radiation with the explored microbiological objects by detects of the changes of amplitudes and durations of scattered light impulses. Under the influence from 0.01 to 10 mM of FeSO4 the maximum of cells' size distribution changed from 0.55 to 0.62 μm and effect of investigated range of FeCl₃×6H₂O concentrations caused it changes from 0.43 to 0.49 μm during five days of sulfurreducing Desulfuromonas acetoxidans bacteria growth.

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Oleksandr I. Bilyy, Oresta M. Vasyliv, and Svitlana O. Hnatush "Light scattering spectra of sulfur-reducing bacteria Desulfuromonas acetoxidans under the influence of ions Fe metals", Proc. SPIE 8338, Tenth International Conference on Correlation Optics, 83381I (22 November 2011); https://doi.org/10.1117/12.916150

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