A label-free antigen-antibody immunosensor based on a special double cladding fiber

Wenxin Cao; Fufei Pang; Na Chen; Zhenyi Chen; Tingyun Wang

doi:10.1117/12.975260

7 November 2012 A label-free antigen-antibody immunosensor based on a special double cladding fiber

Wenxin Cao, Fufei Pang, Na Chen, Zhenyi Chen, Tingyun Wang

Proceedings Volume 8421, OFS2012 22nd International Conference on Optical Fiber Sensors; 84215V (2012) https://doi.org/10.1117/12.975260
Event: OFS2012 22nd International Conference on Optical Fiber Sensor, 2012, Beijing, China

Abstract

An optical fiber label-free antigen-antibody immunosensor was proposed and demonstrated. It was fabricated by a special double cladding fiber (DCF) which presents a band-rejection resonant transmission spectrum. With the 3- aminopropyltriethoxysilane (KH-550) as the crosslink agent, goat anti rabbit IgG was immobilized on the surface of the DCF sensor. The whole process of immobilization and the antigen-antibody reaction was monitored through the shifts of the cladding mode resonant spectrum. Experimental results show that the resonant spectrum shifted toward longer wavelength during antigen-antibody reaction process. And the spectrum shift presented a saturation trend at high concentration rabbit IgG.

Citation Download Citation

Wenxin Cao, Fufei Pang, Na Chen, Zhenyi Chen, and Tingyun Wang "A label-free antigen-antibody immunosensor based on a special double cladding fiber", Proc. SPIE 8421, OFS2012 22nd International Conference on Optical Fiber Sensors, 84215V (7 November 2012); https://doi.org/10.1117/12.975260

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