Investigation of the mechanical property of individual cell using axial optical tweezers

Mary-Clare Dy; Tadao Sugiura; Kotaro Minato

doi:10.1117/12.2003092

22 February 2013 Investigation of the mechanical property of individual cell using axial optical tweezers

Mary-Clare Dy, Tadao Sugiura, Kotaro Minato

Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 85871U (2013) https://doi.org/10.1117/12.2003092
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Optical tweezers is a technique that can trap and manipulate small objects using a highly focused laser beam. Because optical tweezers can also be used to measure small forces, it has been extensively used for the measurement of the mechanical forces of cells. Previous research works typically study particle manipulation and cell force measurement in the lateral direction, hence excluding valuable insights about the axial mechanical properties of cells. Other works that investigate axial cell force measurements utilize spatial light modulators and other devices that are expensive and complicate the setup. Thus, in our study, we designed a simple scheme that can axially manipulate particles by adjusting the position of one lens, called L1-lens, in our setup. Image processing techniques were utilized to determine the changes in the axial particle translation, providing nanometer sensitivity. We investigated the capability of our system using two different-sized particles and results show that for a given L1-lens default position and movement, a 2-micron particle and a 4.26-micron particle were moved axially for 7.68 µm and 4.83 µm, respectively. Axial trapping stiffness was also measured for the stated bead sizes in different magnification. Using the computed trapping sti_ness, we will investigate the axial reactive forces of cells.

Citation Download Citation

Mary-Clare Dy, Tadao Sugiura, and Kotaro Minato "Investigation of the mechanical property of individual cell using axial optical tweezers", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871U (22 February 2013); https://doi.org/10.1117/12.2003092

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Particles

Optical tweezers

Image processing

Microscopes

Objectives

Calibration

Image analysis

Show All Keywords

Keywords/Phrases

Search In:

Publication Years