Adaptation of commercial microscopes for advanced imaging applications

Craig Brideau; Kelvin Poon; Peter Stys

doi:10.1117/12.2079625

5 March 2015 Adaptation of commercial microscopes for advanced imaging applications

Craig Brideau, Kelvin Poon, Peter Stys

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 93292U (2015) https://doi.org/10.1117/12.2079625
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Today’s commercially available microscopes offer a wide array of options to accommodate common imaging experiments. Occasionally, an experimental goal will require an unusual light source, filter, or even irregular sample that is not compatible with existing equipment. In these situations the ability to modify an existing microscopy platform with custom accessories can greatly extend its utility and allow for experiments not possible with stock equipment. Light source conditioning/manipulation such as polarization, beam diameter or even custom source filtering can easily be added with bulk components. Custom and after-market detectors can be added to external ports using optical construction hardware and adapters. This paper will present various examples of modifications carried out on commercial microscopes to address both atypical imaging modalities and research needs. Violet and near-ultraviolet source adaptation, custom detection filtering, and laser beam conditioning and control modifications will be demonstrated. The availability of basic ‘building block’ parts will be discussed with respect to user safety, construction strategies, and ease of use.

Citation Download Citation

Craig Brideau, Kelvin Poon, and Peter Stys "Adaptation of commercial microscopes for advanced imaging applications", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93292U (5 March 2015); https://doi.org/10.1117/12.2079625

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KEYWORDS

Optical filters

Lamps

Microscopes

Control systems

Head

Confocal microscopy

Sensors

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