Portable and modularized fluorometer based on optical fiber

WeiWei Yue; Lei Zhang; ZhenYa Guo; ShouZhen Jiang; ChengJie Bai

doi:10.1117/12.2182022

6 March 2015 Portable and modularized fluorometer based on optical fiber

WeiWei Yue, Lei Zhang, ZhenYa Guo, ShouZhen Jiang, ChengJie Bai

Proceedings Volume 9446, Ninth International Symposium on Precision Engineering Measurement and Instrumentation; 94464I (2015) https://doi.org/10.1117/12.2182022
Event: International Symposium on Precision Engineering Measurement and Instrumentation, 2014, Changsha/Zhangjiajie, China

Abstract

A portable and modularized fluorometer based on optical fiber was proposed in this work. The fluorometer included a light emitter diode (LED) light source module (LSM), a sample cell module (SCM), an optical-electrical converter module (OCM) and a signal process module (SAM). The LEDs in LSM were driven by a constant current source to provide stable exciting light with different wavelength. The OCM included a modularized optical filter and used a photomultiplier tube (PMT) to detect fluorescence signal. The SCM was used to locate sample cuvette and could be connected by optical fibers with the LSM and OCM. Via modularized design, the LSM and OCM could both selected and replaced based on different fluorescence dyes. In order to improve the detecting dynamic range of the fluorometer, the SAM could control the light intensity of LED source in LSM, to control the gain of PMT in OCM, and particularly, four channel signal acquisition circuits with different gain were constructed to collect fluorescence signal simultaneously. Fluorescein isothiocyanate (FITC) was selected as sample to test the fluorometer. The fluorometer has shown a high sensitivity with FITC concentration of 10ng/mL and presented a good linearity from 10 ng/mL to 10 μg/mL.

Citation Download Citation

WeiWei Yue, Lei Zhang, ZhenYa Guo, ShouZhen Jiang, and ChengJie Bai "Portable and modularized fluorometer based on optical fiber", Proc. SPIE 9446, Ninth International Symposium on Precision Engineering Measurement and Instrumentation, 94464I (6 March 2015); https://doi.org/10.1117/12.2182022

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available