Holographic 3D fluorescence microscopy

David C. Clark; Changwon Jang; Jonghyun Kim; Byoungho Lee; Myung K. Kim

doi:10.1117/12.2185087

1 July 2015 Holographic 3D fluorescence microscopy

David C. Clark, Changwon Jang, Jonghyun Kim, Byoungho Lee, Myung K. Kim

Proceedings Volume 9655, Fifth Asia-Pacific Optical Sensors Conference; 96551M (2015) https://doi.org/10.1117/12.2185087
Event: Fifth Asia Pacific Optical Sensors Conference, 2015, Jeju, Korea, Republic of

Abstract

Fluorescence microscopy is an indispensable imaging tool in modern biomedical research. Holography is well-known to have many interesting and useful imaging capabilities. But the requirement of coherent illumination has all but precluded holography as a means for fluorescence imaging, which is inherently incoherent. Recent developments in digital holography, however, including self-interference incoherent digital holography (SIDH), provide highly effective and versatile capabilities for 3D holographic imaging with incoherent light, that can remove the barrier between fluorescence and holography. Recent progress in holographic fluorescence microscopy is presented.

Citation Download Citation

David C. Clark, Changwon Jang, Jonghyun Kim, Byoungho Lee, and Myung K. Kim "Holographic 3D fluorescence microscopy", Proc. SPIE 9655, Fifth Asia-Pacific Optical Sensors Conference, 96551M (1 July 2015); https://doi.org/10.1117/12.2185087

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KEYWORDS

Holograms

Holography

Luminescence

Microscopy

3D image reconstruction

Digital holography

3D image processing

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