Dynamic speckle study of microbial growth

F. M. Vincitorio; C. Mulone; P. A. Marcuzzi; N. Budini; C. Freyre; A. J. Lopez; A. Ramil

doi:10.1117/12.2196257

24 August 2015 Dynamic speckle study of microbial growth

F. M. Vincitorio, C. Mulone, P. A. Marcuzzi, N. Budini, C. Freyre, A. J. Lopez, A. Ramil

Proceedings Volume 9660, SPECKLE 2015: VI International Conference on Speckle Metrology; 96601Q (2015) https://doi.org/10.1117/12.2196257
Event: SPECKLE 2015: VI International Conference on Speckle Metrology, 2015, Guanajuato, Mexico

Abstract

In this work we present a characterization of yeast dynamic speckle activity during growth in an isolated agar culture medium. We found that it is possible to detect the growth of the microorganisms even before they turn out to be visible. By observing the time evolution of the speckle activity at different regions of the culture medium we could extract a map of the growth process, which served to analyze how the yeast develops and spreads over the agar's medium. An interesting point of this study concerns with the influence of the laser light on the yeast growth rate. We have found that yeast finds hard to develop at regions with higher laser light illumination, although we used a synchronous system to capture the speckle pattern. The results obtained in this work would serve us as a starting point to fabricate a detector of growing microorganism colonies, with obvious interesting applications in diverse areas.

Citation Download Citation

F. M. Vincitorio, C. Mulone, P. A. Marcuzzi, N. Budini, C. Freyre, A. J. Lopez, and A. Ramil "Dynamic speckle study of microbial growth ", Proc. SPIE 9660, SPECKLE 2015: VI International Conference on Speckle Metrology, 96601Q (24 August 2015); https://doi.org/10.1117/12.2196257

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available