The study on RBC characteristic in paroxysmal nocturnal hemoglobinuria (PNH) patients using common path interferometric quantitative phase microscopy

Byung Jun Park; Youngjae Won; Byungyeon Kim; Seungrag Lee

doi:10.1117/12.2210873

9 March 2016 The study on RBC characteristic in paroxysmal nocturnal hemoglobinuria (PNH) patients using common path interferometric quantitative phase microscopy

Byung Jun Park, Youngjae Won, Byungyeon Kim, Seungrag Lee

Author Affiliations +

Proceedings Volume 9718, Quantitative Phase Imaging II; 971819 (2016) https://doi.org/10.1117/12.2210873
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We have studied the RBC membrane properties between a normal RBC and a RBC in Paroxysrnal nocturnal hemoglobinuria (PNH) patient using common path interferometric quantitative phase microscopy (CPIQPM). CPIQPM system has provided the subnanometer optical path length sensitivity on a millisecond. We have measured the dynamic thickness fluctuations of a normal RBC membrane and a RBC membrane in PNH patient over the whole cell surface with CPIQPM. PNH is a rare and serious disease of blood featured by destruction of red blood cells (RBCs). This destruction happens since RBCs show the defect of protein which protects RBCs from the immune system. We have applied CPIQPM to study the characteristic of RBC membrane in PNH patient. We have shown the morphological shape, volume, and projected surface for both different RBC types. The results have showed both RBCs had the similar shape with donut, but membrane fluctuations in PNH patient was shown to reveal the difference of temporal properties compared with a normal RBC. In order to demonstrate the practical tool of the CPIQPM technique, we have also obtained the time series thickness fluctuation outside a cell.

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Byung Jun Park, Youngjae Won, Byungyeon Kim, and Seungrag Lee "The study on RBC characteristic in paroxysmal nocturnal hemoglobinuria (PNH) patients using common path interferometric quantitative phase microscopy", Proc. SPIE 9718, Quantitative Phase Imaging II, 971819 (9 March 2016); https://doi.org/10.1117/12.2210873

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KEYWORDS

Microscopy

Blood

Interferometry

Phase imaging

Phase interferometry

3D image processing

Edge detection

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