Quantitative phase imaging of cell division in yeast cells and E.coli using digital holographic microscopy

Vimal Prabhu Pandiyan; Renu John

doi:10.1117/12.2207425

9 December 2015 Quantitative phase imaging of cell division in yeast cells and E.coli using digital holographic microscopy

Vimal Prabhu Pandiyan, Renu John

Author Affiliations +

Proceedings Volume 9792, Biophotonics Japan 2015; 97920Y (2015) https://doi.org/10.1117/12.2207425
Event: SPIE/OSJ Biophotonics Japan, 2015, Tokyo, Japan

Abstract

Digital holographic microscope (DHM) is an emerging quantitative phase imaging technique with unique imaging scales and resolutions leading to multitude of applications. DHM is promising as a novel investigational and applied tool for cell imaging, studying the morphology and real time dynamics of cells and a number of related applications. The use of numerical propagation and computational digital optics offer unique flexibility to tune the depth of focus, and compensate for image aberrations. In this work, we report imaging the dynamics of cell division in E.coli and yeast cells using a DHM platform. We demonstrate 3-D and depth imaging as well as reconstruction of phase profiles of E.coli and yeast cells using the system. We record a digital hologram of E.coli and yeast cells and reconstruct the image using Fresnel propagation algorithm. We also use aberration compensation algorithms for correcting the aberrations that are introduced by the microscope objective in the object path using linear least square fitting techniques. This work demonstrates the strong potential of a DHM platform in 3-D live cell imaging, fast clinical quantifications and pathological applications.

Citation Download Citation

Vimal Prabhu Pandiyan and Renu John "Quantitative phase imaging of cell division in yeast cells and E.coli using digital holographic microscopy", Proc. SPIE 9792, Biophotonics Japan 2015, 97920Y (9 December 2015); https://doi.org/10.1117/12.2207425

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Digital holography

3D image reconstruction

Holograms

Yeast

Holography

Microscopy

Digital imaging

Show All Keywords

Keywords/Phrases

Search In:

Publication Years