We have developed Polychromatic Digital Holographic Microscopy (P-DHM), providing quasi coherent noise-free OPD images, but with some limitations regarding the acquisition and reconstruction time of the multi-wavelength holograms. Our recent work, enabling a fully automated multi-wavelength hologram reconstruction associated with an increased acquisition speed, achieving a frame rate between 6 and 15 fps, while preserving OPD high sensitivity, have led to a P-DHM efficient for live-cell imaging. In addition to these technical developments, appealing applications such as the exploration of intracellular trafficking, cell migration, and neurite evolution achieved with P-DHM will be presented.
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