Dr. Matthias Patting Profile

Dr. Matthias Patting

Senior Scientist at PicoQuant GmbH

SPIE Involvement:

Author

Publications (26)

Proceedings Article | 13 March 2024 Presentation

Presentation

Bringing time-resolved fluorescence microscopy to a broader research community

Felix Koberling, Fabio Barachati, Marcelle Koenig, Maria Loidolt-Krueger, Ellen Schmeyer, Matthias Patting, Marcus Sackrow, Uwe Ortmann, Evangelos Sisamakis, Rainer Erdmann

Proceedings Volume PC12847, PC1284708 (2024) https://doi.org/10.1117/12.3007783

KEYWORDS: Confocal microscopy, Fluorescence microscopy, Fluorescence lifetime imaging, Imaging technologies, Cameras, Video, Time resolved spectroscopy, Time correlated single photon counting, Sampling rates, Picosecond phenomena

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Proceedings Article | 13 March 2024 Presentation

Presentation

Luminosa: making confocal fluorescence microscopy a tool for every biophysicist

Felix Koberling, Fabio Barachati, Marcelle Koenig, Maria Loidolt-Krueger, Ellen Schmeyer, Matthias Patting, Marcus Sackrow, Uwe Ortmann, Evangelos Sisamakis, Rainer Erdmann

Proceedings Volume PC12847, PC1284710 (2024) https://doi.org/10.1117/12.3006371

KEYWORDS: Confocal microscopy, Fluorescence microscopy, Fluorescence lifetime imaging, Statistical analysis, Image analysis, Time resolved spectroscopy, Statistical modeling, Reproducibility, Process modeling, Photon counting

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Proceedings Article | 13 March 2024 Presentation

Presentation

Fast analysis with minimal user interaction in fluorescence lifetime imaging

Felix Koberling, Fabio Barachati, Marcelle König, Maria Loidolt-Krueger, Ellen Schmeyer, Matthias Patting, Marcus Sackrow, Uwe Ortmann, Evangelos Sisamakis, Rainer Erdmann

Proceedings Volume PC12849, PC128490N (2024) https://doi.org/10.1117/12.3001448

KEYWORDS: Fluorescence lifetime imaging, Statistical analysis, Analytical research, Statistical modeling, Process modeling, Multiplexing, Microscopes, Detection and tracking algorithms, Confocal microscopy

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Proceedings Article | 17 March 2023 Poster

Poster

Fast analysis with minimal user interaction in Fluorescence Lifetime Imaging

Fabio Barachati, Marcelle König, Maria Loidolt-Krüger, Ellen Schmeyer, Matthias Patting, Marcus Sackrow, Uwe Ortmann, Felix Koberling, Rainer Erdmann

Proceedings Volume PC12386, PC123860L (2023) https://doi.org/10.1117/12.2651118

KEYWORDS: Fluorescence lifetime imaging, Statistical modeling, Statistical analysis, Sensors, Process modeling, Multiplexing, Microscopes, Detection and tracking algorithms, Confocal microscopy, Analytical research

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Proceedings Article | 15 March 2023 Presentation

Presentation

Easy and reliable way to perform single molecule FRET measurements (Conference Presentation)

Felix Koberling, Marcelle König, Maria Loidolt-Krüger, Fabio Barachati, Matthias Patting, Marcus Sackrow, Kamil Bobowski, Mathias Bayer, Rainer Erdmann

Proceedings Volume PC12386, PC1238603 (2023) https://doi.org/10.1117/12.2649871

KEYWORDS: Molecules, Fluorescence resonance energy transfer, Proteins, Point spread functions, Photonic microstructures, Microscopes, Confocal microscopy

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Proceedings Article | 3 March 2022 Presentation

Presentation

Measuring steady-state and time-resolved photoluminescence of up-conversion nanoparticles with high spatial resolution

Christian Oelsner, Linnea Olofsson, Uwe Ortmann, Eugeny Ermilov, Volker Buschmann, Matthias Patting, Rainer Erdmann

Proceedings Volume PC11976, PC1197601 (2022) https://doi.org/10.1117/12.2605888

KEYWORDS: Nanoparticles, Spatial resolution, Luminescence, Spectroscopy, Visible radiation, Time resolved spectroscopy, Resonance energy transfer, Optical properties, Near infrared, Molecular interactions

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Proceedings Article | 2 March 2022 Poster

Poster

Fluorescence lifetime image scanning microscopy

Ingo Gregor, Niels Rademacher, Max Tillmann, Matthias Patting, Jörg Enderlein, Felix Koberling

Proceedings Volume PC11967, PC119670J (2022) https://doi.org/10.1117/12.2625458

KEYWORDS: Microscopy, Luminescence, Single photon detectors, Microscopes, Fluorescence lifetime imaging, Confocal microscopy, Tissues, Spatial resolution, Single photon, Sensors

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Proceedings Article | 5 March 2021 Presentation

Presentation

Visualizing dynamic processes with rapidFLIMHiRes, the ultra fast FLIM imaging method with outstanding 10ps time resolution

Maria Loidolt-Krüger, Fabian Jolmes, Matthias Patting, Michael Wahl, Evangelos Sismakis, André Devaux, Uwe Ortmann, Felix Koberling, Rainer Erdmann

Proceedings Volume 11654, 116540I (2021) https://doi.org/10.1117/12.2583684

KEYWORDS: Fluorescence lifetime imaging, Visualization, Ultrafast imaging, Image resolution, Temporal resolution, Sensors, Picosecond phenomena, Fluorescence resonance energy transfer, Tissues, Single photon

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Proceedings Article | 5 March 2021 Presentation

Presentation

Visualizing dynamic processes with rapidFLIMHiRes, the ultra fast FLIM imaging method with outstanding 10ps time resolution

Maria Loidolt-Krüger, Fabian Jolmes, Matthias Patting, Michael Wahl, Evangelos Sismakis, André Devaux, Uwe Ortmann, Felix Koberling, Rainer Erdmann

Proceedings Volume 11648, 116480D (2021) https://doi.org/10.1117/12.2583477

KEYWORDS: Fluorescence lifetime imaging, Visualization, Ultrafast imaging, Image resolution, Temporal resolution, Sensors, Picosecond phenomena, Fluorescence resonance energy transfer, Tissues, Single photon

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Proceedings Article | 5 March 2021 Presentation

Presentation

Measuring steady-state and time-resolved photoluminescence from a positionable, micrometer-sized observation volume

Christian Oelsner, Eugeny Ermilov, Volker Buschmann, Frank Birke, Matthias Patting, Uwe Ortmann, Rainer Erdmann

Proceedings Volume 11656, 116560H (2021) https://doi.org/10.1117/12.2576400

KEYWORDS: Luminescence, Spectroscopy, Statistical analysis, Molecules, Molecular spectroscopy, Time resolved spectroscopy, Spatial resolution, Semiconductors, Resonance energy transfer, Proteins

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Proceedings Article | 4 March 2019 Presentation

Presentation

Spectrally resolved and high speed TCSPC-based fluorescence lifetime imaging (Conference Presentation)

Tino Roehlicke, Matthias Patting, Hans-Juergen Rahn, Benedikt Kraemer, Michael Wahl, Felix Koberling, Rainer Erdmann

Proceedings Volume 10889, 108890Q (2019) https://doi.org/10.1117/12.2513895

KEYWORDS: Fluorescence lifetime imaging, Signal detection, Sensors, Live cell imaging, Single photon, Luminescence, Electronics, Image acquisition, Picosecond phenomena, Confocal microscopy

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Proceedings Article | 4 March 2019 Presentation

Presentation

Quantitative ultra-fast FLIM and lifetime based multi-species analysis (Conference Presentation)

Benedikt Kraemer, Sumeet Rohilla, Rhys Dowler, Marcus Sackrow, Matthias Patting, Hans-Jürgen Rahn, Michael Wahl, Uwe Ortmann, Felix Koberling, Andreas Hocke, Rainer Erdmann

Proceedings Volume 10882, 108820K (2019) https://doi.org/10.1117/12.2507396

KEYWORDS: Fluorescence lifetime imaging, Ultrafast phenomena, Tissues, Image processing, Luminescence, Confocal microscopy, Life sciences, Photomultipliers, Sensors, Spatial resolution

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Proceedings Article | 14 March 2018 Presentation

Presentation

Scanning FCS and multi-species STED microscopy for diffusion studies in membranes (Conference Presentation)

Felix Koberling, Mariano Gonzalez Pisfil, Rhys Dowler, Marcelle Koenig, Benedikt Kraemer, Paja Reisch, Matthias Patting

Proceedings Volume 10500, 1050006 (2018) https://doi.org/10.1117/12.2289677

KEYWORDS: Fluorescence correlation spectroscopy, Stimulated emission depletion microscopy, Diffusion, Molecules, Microscopy, Molecular spectroscopy, Confocal microscopy, Molecular interactions, Microscopes, Single photon

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SPIE Journal Paper | 22 January 2018

Fluorescence decay data analysis correcting for detector pulse pile-up at very high count rates

Matthias Patting, Paja Reisch, Marcus Sackrow, Rhys Dowler, Marcelle Koenig, Michael Wahl

OE, Vol. 57, Issue 03, 031305, (January 2018) https://doi.org/10.1117/12.10.1117/1.OE.57.3.031305

KEYWORDS: Sensors, Luminescence, Fluorescence lifetime imaging, Data analysis, Error analysis, Photodetectors, Single photon, Confocal microscopy, Electronics, Time correlated photon counting

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Proceedings Article | 14 March 2016 Paper

Paper

ns-time resolution for multispecies STED-FLIM and artifact free STED-FCS

Marcelle Koenig, Paja Reisch, Rhys Dowler, Benedikt Kraemer, Sebastian Tannert, Matthias Patting, Mathias Clausen, Silvia Galiani, Christian Eggeling, Felix Koberling, Rainer Erdmann

Proceedings Volume 9712, 97120T (2016) https://doi.org/10.1117/12.2208874

KEYWORDS: Fluorescence correlation spectroscopy, Super resolution, Microscopy, Pulsed laser operation, Luminescence, Picosecond phenomena, Stimulated emission depletion microscopy, Optical resolution, Time correlated photon counting, Single photon, Diffusion, Confocal microscopy, Molecules, Diffraction

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Proceedings Article | 26 February 2010 Paper

Paper

Fast rasterscanning enables FLIM in macroscopic samples up to several centimeters

F. Koberling, V. Buschmann, C. Hille, M. Patting, C. Dosche, A. Sandberg, A. Wheelock, R. Erdmann

Proceedings Volume 7569, 756931 (2010) https://doi.org/10.1117/12.843453

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Scanners, Proteins, Microscopes, Single photon, Sensors, Laser scanners, Multiplexing, Imaging systems

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Proceedings Article | 25 February 2009 Paper

Paper

Recent advances in photon coincidence measurements for photon antibunching and full correlation analysis

F. Koberling, B. Kraemer, V. Buschmann, S. Ruettinger, P. Kapusta, M. Patting, M. Wahl, R. Erdmann

Proceedings Volume 7185, 71850Q (2009) https://doi.org/10.1117/12.807803

KEYWORDS: Molecules, Signal detection, Sensors, Picosecond phenomena, Luminescence, Fluorescence correlation spectroscopy, Diffusion, Confocal microscopy, Electronics, Time metrology

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Proceedings Article | 25 February 2009 Paper

Paper

Comparison of background corrected fluorescence correlation spectroscopy and fluorescence lifetime correlation spectroscopy: dilution series revisited

Steffen Rüttinger, Peter Kapusta, Matthias Patting, Michael Wahl, Rainer Macdonald

Proceedings Volume 7185, 718508 (2009) https://doi.org/10.1117/12.808883

KEYWORDS: Fluorescence correlation spectroscopy, Sensors, Luminescence, Photons, Confocal microscopy, Scattering, Fluorescence spectroscopy, Spectroscopy, Light scattering, Signal detection

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Proceedings Article | 21 February 2008 Paper

Paper

Recent advances in time-correlated single-photon counting

Felix Koberling, Benedikt Krämer, Sebastian Tannert, Steffen Rüttinger, Uwe Ortmann, Matthias Patting, Michael Wahl, Benjamin Ewers, Peter Kapusta, Rainer Erdmann

Proceedings Volume 6862, 686209 (2008) https://doi.org/10.1117/12.761161

KEYWORDS: Fluorescence correlation spectroscopy, Photons, Luminescence, Sensors, Diffusion, Pulsed laser operation, Picosecond phenomena, Confocal microscopy, Molecules, Light scattering

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Proceedings Article | 15 February 2008 Paper

Paper

Advanced FRET and FCS measurements with laser scanning microscopes based on time-resolved techniques

B. Krämer, V. Buschmann, U. Ortmann, F. Koberling, M. Wahl, M. Patting, P. Kapusta, A. Bülter, R. Erdmann

Proceedings Volume 6860, 68601D (2008) https://doi.org/10.1117/12.761141

KEYWORDS: Sensors, Luminescence, Fluorescence correlation spectroscopy, Fluorescence resonance energy transfer, Pulsed laser operation, Laser scanners, Microscopes, Single photon, Data acquisition, Fluorescence lifetime imaging

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Proceedings Article | 11 May 2007 Paper

Paper

Advanced TCSPC electronics and their use in general quantum physics and demanding analytical applications

Michael Wahl, Hans-Juergen Rahn, Matthias Patting, Felix Koberling, Rainer Erdmann

Proceedings Volume 6583, 658306 (2007) https://doi.org/10.1117/12.722799

KEYWORDS: Photons, Luminescence, Picosecond phenomena, Molecules, Electronics, Sensors, Time metrology, Fluorescence correlation spectroscopy, Fluorescence lifetime imaging, Quantum physics

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Proceedings Article | 11 May 2007 Paper

Paper

Dead-time effects in TCSPC data analysis

Matthias Patting, Michael Wahl, Peter Kapusta, Rainer Erdmann

Proceedings Volume 6583, 658307 (2007) https://doi.org/10.1117/12.722804

KEYWORDS: Data modeling, Photodetectors, Picosecond phenomena, Sensors, Data analysis, Time correlated photon counting, Error analysis, Luminescence, Single photon, Monochromators

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Proceedings Article | 11 May 2007 Paper

Paper

Time-resolved confocal fluorescence microscopy: novel technical features and applications for FLIM, FRET and FCS using a sophisticated data acquisition concept in TCSPC

Felix Koberling, Benedikt Krämer, Peter Kapusta, Matthias Patting, Michael Wahl, Rainer Erdmann

Proceedings Volume 6583, 65830Y (2007) https://doi.org/10.1117/12.722867

KEYWORDS: Fluorescence resonance energy transfer, Luminescence, Molecules, Fluorescence correlation spectroscopy, Confocal microscopy, Fluorescence lifetime imaging, Data acquisition, Molecular photonics, Microscopy, Picosecond phenomena

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In recent years time-resolved fluorescence measurement and analysis techniques became a standard in single molecule microscopy. However, considering the equipment and experimental implementation they are typically still an add-on and offer only limited possibilities to study the mutual dependencies with common intensity and spectral information. In contrast, we are using a specially designed instrument with an unrestricted photon data acquisition approach which allows to store spatial, temporal, spectral and intensity information in a generalized format preserving the full experimental information. This format allows us not only to easily study dependencies between various fluorescence parameters but also to use, for example, the photon arrival time for sorting and weighting the detected photons to improve the significance in common FCS and FRET analysis schemes. The power of this approach will be demonstrated for different techniques: In FCS experiments the concentration determination accuracy can be easily improved by a simple time-gated photon analysis to suppress the fast decaying background signal. A more detailed analysis of the arrival times allows even to separate FCS curves for species which differ in their fluorescence lifetime but, for example, cannot be distinguished spectrally. In multichromophoric systems like a photonic wire which undergoes unidirectional multistep FRET the lifetime information complements significantly the intensity based analysis and helps to assign the respective FRET partners. Moreover, together with pulsed excitation the time-correlated analysis enables directly to take advantage of alternating multi-colour laser excitation. This pulsed interleaved excitation (PIE) can be used to identify and rule out inactive FRET molecules which cause interfering artefacts in standard FRET efficiency analysis. We used a piezo scanner based confocal microscope with compact picosecond diode lasers as excitation sources. The timing performance can be significantly increased by using new SPAD detectors which enable, in conjunction with new TCSPC electronics, an overall IRF width of less than 120 ps maintaining single molecule sensitivity.

Proceedings Article | 27 February 2006 Paper

Paper

Identification of triplet state and photoisomerization of Cy5 by means of ensemble and single-molecule measurements

Zhengxi Huang, Dongmei Ji, Sufan Wang, Andong Xia, Felix Koberling, Matthias Patting, Rainer Erdmann

Proceedings Volume 6092, 60920Y (2006) https://doi.org/10.1117/12.650492

KEYWORDS: Molecules, Luminescence, Absorption, Phosphorescence, Bioalcohols, Molecular lasers, Photolysis, Chemical species, Glasses, Spectrophotometry

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Proceedings Article | 18 June 2004 Paper

Paper

Compact TCSPC upgrade package for laser scanning microscopes based on 375- to 470-nm picosecond diode lasers

Uwe Ortmann, T. Dertinger, Michael Wahl, Matthias Patting, Rainer Erdmann

Proceedings Volume 5325, (2004) https://doi.org/10.1117/12.536888

KEYWORDS: Laser scanners, Luminescence, Sensors, Semiconductor lasers, Picosecond phenomena, Data acquisition, Microscopes, Photon counting, Signal detection, Fluorescence lifetime imaging

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We present the technical integration of state-of-the-art picosecond diode laser sources and data acquisition electronics in conventional laser scanning microscopes. This offers users of laser scanning microscopes an easy upgrade path towards time-resolved measurements. Our setup uses picosecond diode lasers from 375 nm, 405 nm, 440 nm and 470 nm for fluorescence excitation which are coupled in through a sole single mode fiber. The detected signal is guided to a photon counting detector, such as Photomultiplier Tubes (PMT) or Single Photon Avalanche Diodes (SPAD). This combines the outstanding sensitivity of photon counting detectors with the ease of use of diode laser sources, to allow time-resolved measurements of fluorescence decays with resolutions down to picoseconds. The synchronization signals from the laser scanning microscope are fed into the data stream recorded by the TimeHarp 200 TCSPC5,7 system, via the unique Time-Tagged Time-Resolved (TTTR)6 data acquisition mode. In this TTTR data acquisition mode each photon is recorded individually with its specific parameters as detector channel, picosecond timing, global arrival time and, in this special application, up to three additional markers. These markers, in combination with the global arrival time, allow the system software to reconstruct the complete image and subsequently fit the full fluorescence lifetime image. The multi-parameter data acquisition scheme of the TimeHarp 200 electronics not only records each parameter individually, but offers in addition the opportunity to analyse the parameter dependencies in a multitude of different ways. This method allows not only to calculate the fluorescence fluctuation correlation function (FCS) on any single spot of interest but also to reconstruct the fluorescence decay of each image pixel and detector channel for the purpose of Fluorescence Lifetime Imaging (FLIM) or advanced Fluorescence Resonance Energy Transfer (FRET) analysis. We present here some selected results acquired with standard laser scanning microscopes upgraded for the time-correlated single photon counting technique.

Proceedings Article | 14 October 2003 Paper

Paper

Two-channel fluorescence lifetime microscope with two colour laser excitation, single-molecule sensitivity, and submicrometer resolution

Felix Koberling, Michael Wahl, Matthias Patting, Hans-Juergen Rahn, Peter Kapusta, Rainer Erdmann

Proceedings Volume 5143, (2003) https://doi.org/10.1117/12.500610

KEYWORDS: Luminescence, Molecules, Microscopes, Fluorescence lifetime imaging, Sensors, Fluorescence correlation spectroscopy, Polarization, Picosecond phenomena, Single photon, Electronics

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Showing 5 of 26 publications

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