Mr. Zheng Shi Profile

Zheng Shi

at Zhejiang Univ

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Proceedings Article | 9 January 2019 Paper

In-site chlorophyll-a fluorometer based on lock-in amplifier

Zheng Shi, Ya Gao, Yingtian Hu, Chao Liu, Xiaoping Wang

Proceedings Volume 10850, 1085014 (2019) https://doi.org/10.1117/12.2505482

KEYWORDS: Sensors, Luminescence, Optical amplifiers, Light emitting diodes, Oceanography, Water, Fluorescence spectroscopy, Light sources, Fluorometers, Photodiodes

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The content of chlorophyll-a is one of the important indicators of eutrophication in marine ranching. Accurate measurement of chlorophyll-a content is of great significance for the sustainable development of marine ranching. In order to realize insite detection of chlorophyll-a content in marine ranching, a chlorophyll-a fluorometer based on lock-in amplifier was designed according to the fluorescence characteristics of chlorophyll-a. A high-brightness 460nm LED was used as the excitation source and the chlorophyll-a fluorescence was detected at 680nm by a photodiode through an interference filter. At the same time, a reference light path was used to compensate the change of light source. The response of the sensor was tested using chlorophyll-a solution, the results showed the sensor has good linearity in the dynamic range and the correlation coefficient is 0.996. The noise level was tested using pure water, the test results showed that the sensor has good noise performance and the resolution of the sensor is 0.2μg/L.

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