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Imaging the spatial and temporal effects of millisecond duration pulses of infrared light on neurons requires image frame rates approaching 1000+ Hz to capture neural activity. Autofluorescence imaging of the metabolic coenzymes reduced nicotinamide adenine dinucleotide and flavin adenine dinucleotide provides information about cellular metabolism and can be a surrogate measurement of neural activity. Here, we are combining fast fluorescence microscopy techniques with modeling and machine learning to image autofluorescence dynamics in cells following exposure to infrared light.
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Alex J. Walsh, Jocelyn Martinez, Anna Theodossiou, "High speed autofluorescence imaging of the effects of short pulses of infrared light," Proc. SPIE PC11971, High-Speed Biomedical Imaging and Spectroscopy VII, PC119710B (2 March 2022); https://doi.org/10.1117/12.2613561