Multi-lightsheet RESOLFT for volumetric imaging beyond the diffraction limit (Conference Presentation)

Andreas Bodén; Ilaria Testa

doi:10.1117/12.2668403

15 March 2023 Multi-lightsheet RESOLFT for volumetric imaging beyond the diffraction limit (Conference Presentation)

Andreas Bodén, Ilaria Testa

Proceedings Volume PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; PC1238506 (2023) https://doi.org/10.1117/12.2668403
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Single-Objective Light Sheet (SOLS) microscopes have in recent years emerged as a viable option for rapid and gentle three-dimensional imaging of biological samples. Though ingenious tricks have pushed the optical resolution of such systems to its physical limits, diffraction still forces a bothersome trade-off between light sheet thickness and propagation length. In this work we show that Reversibly Switchable Fluorescent Proteins (RSFPs) can be used to overcome this limit on light sheet thickness allowing fast volumetric imaging with approximately five to ten thinner effective light sheets compared to diffraction limited approaches. This volumetrically parallelized acquisition scheme opens up new possibilities for super-resolution imaging.

Conference Presentation

Citation Download Citation

Andreas Bodén and Ilaria Testa "Multi-lightsheet RESOLFT for volumetric imaging beyond the diffraction limit (Conference Presentation)", Proc. SPIE PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, PC1238506 (15 March 2023); https://doi.org/10.1117/12.2668403

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