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13 March 2024 Hyperspectral oblique plane microscopy
Christopher J. Rowlands, Ke Guo, Periklis Pantazis, Konstantinos Kalyviotis
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Proceedings Volume PC12853, High-Speed Biomedical Imaging and Spectroscopy IX; PC128530N (2024) https://doi.org/10.1117/12.3003078
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Hyperspectral oblique plane microscopy provides Raman contrast images of biological specimens with unprecedented pixel throughput. Being a light-sheet technique it can capture hundreds of spectra in the time a point scanning system would take to capture a single spectrum. We will demonstrate the utility of this new system by monitoring wound healing in a zebrafish at five minute intervals, Followed by spontaneous Raman imaging of a beating zebrafish heart.
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Christopher J. Rowlands, Ke Guo, Periklis Pantazis, and Konstantinos Kalyviotis "Hyperspectral oblique plane microscopy", Proc. SPIE PC12853, High-Speed Biomedical Imaging and Spectroscopy IX, PC128530N (13 March 2024); https://doi.org/10.1117/12.3003078
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KEYWORDS
Microscopy
Raman spectroscopy
Laser scattering
Light scattering
Photons
Raman scattering
Spatial resolution
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