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The presence of natural fluorescence in cellular structures and the use of fluorescent probes enables in vivo monitoring of biological and biophysical phenomena with high sensitivity and selectivity. In recent years, the development of on-chip lensless platforms has enabled the development of compact imaging devices. In this work we implement fluorescence detection on a contact, lensless CMOS-based image platform exploring a total internal reflection fluorescence configuration. Our fluorescent imaging platform has the potential to achieve micrometer-scale spatial resolution with a Field of View determined by the size of the semiconductor sensor (in our case 3.68mm x 2.76mm).
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Túlio de Lima Pedrosa, Renato E. Araujo, Sebastian Wachsmann-Hogiu, "Contact-mode fluorescence measurements on a lensless CMOS image sensor," Proc. SPIE PC12858, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XXI, PC1285804 (13 March 2024); https://doi.org/10.1117/12.3002784