Detection of Physiological Levels of Hormonal Steroids Labelled with Organometallic Markers by FT-IR Spectroscopy

Ashraf A. Ismail; Ian S. Butler; Gerard Jaouen; Anne Vessieres; Sylvie Tondu; Siden Top

doi:10.1117/12.970742

20 December 1985 Detection of Physiological Levels of Hormonal Steroids Labelled with Organometallic Markers by FT-IR Spectroscopy

Ashraf A. Ismail, Ian S. Butler, Gerard Jaouen, Anne Vessieres, Sylvie Tondu, Siden Top

Author Affiliations +

Proceedings Volume 0553, Fourier and Computerized Infrared Spectroscopy; (1985) https://doi.org/10.1117/12.970742
Event: 1985 International Conference on Fourier and Computerized Infrared Spectroscopy, 1985, Ottawa, Canada

Abstract

Hormonal steroids such as 17α-estradiol derivatives, labelled with organometallic moieties, have been tested as markers for hormone receptor assay. Among the organometallic labels used were: Cr(C0)3 and Cr(C0)2(CS), which can be attached to the A ring of the steroid, and Co2(C0)6 and Mo2(7-15-05H5)(C0)4, which can be bonded to acetylenic groups in the 1713-position of the five-membered ring of the steroid. The new analytical approach is based on the FT-IR detection of the υ(CO) modes of the organometallic markers. The present estradiol-receptor detection level for 10,000-20,000 scans at 4 cm-1 resolution is about 100 fM, i.e., in the neighbourhood of physiological levels.

Citation Download Citation

Ashraf A. Ismail, Ian S. Butler, Gerard Jaouen, Anne Vessieres, Sylvie Tondu, and Siden Top "Detection of Physiological Levels of Hormonal Steroids Labelled with Organometallic Markers by FT-IR Spectroscopy", Proc. SPIE 0553, Fourier and Computerized Infrared Spectroscopy, (20 December 1985); https://doi.org/10.1117/12.970742

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