Medicinals Bonded To Biopolymers: Quantitative And Qualitative FTIR Determinations

L. G. Tensmeyer; H. L . Hunter; R. Nagarajan; B. Chao; J . B. Deeter; G. A . Koppel; S. L. Briggs

doi:10.1117/12.970761

20 December 1985 Medicinals Bonded To Biopolymers: Quantitative And Qualitative FTIR Determinations

L. G. Tensmeyer, H. L . Hunter, R. Nagarajan, B. Chao, J . B. Deeter, G. A . Koppel, S. L. Briggs

Proceedings Volume 0553, Fourier and Computerized Infrared Spectroscopy; (1985) https://doi.org/10.1117/12.970761
Event: 1985 International Conference on Fourier and Computerized Infrared Spectroscopy, 1985, Ottawa, Canada

Abstract

We report the successful quantitative determination of small molecules (haptens) to biomacromolecules. Results are shown for two such systems: 7-[4-chloro-3,5-dinitro-benzamido)-deacetoxycephalosporanic acid (CDNB-7-ADCA), coupled to bovine serum albumin, and polyethyleneglycol coupled to ovalbumin. In each case, the number of haptens actually bonded is considerably less than theoretically possible. We also report the successful analysis of three additional systems, but examples of their data are not shown: CDNB-7-ADCA,CDNB, and 3-[CDNB]-2-azetidinone coupled to ovalbumin. In all cases, the fact that pertinent moieties of the hapten are intact indicates that the entire hapten is intact also.

Citation Download Citation

L. G. Tensmeyer, H. L . Hunter, R. Nagarajan, B. Chao, J . B. Deeter, G. A . Koppel, and S. L. Briggs "Medicinals Bonded To Biopolymers: Quantitative And Qualitative FTIR Determinations", Proc. SPIE 0553, Fourier and Computerized Infrared Spectroscopy, (20 December 1985); https://doi.org/10.1117/12.970761

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available