Error Analysis Of Phase Resolution Methods For Elimination Of Quenching Effects In Luminescence Spectroscopy

J N Demas

doi:10.1117/12.945456

27 April 1988 Error Analysis Of Phase Resolution Methods For Elimination Of Quenching Effects In Luminescence Spectroscopy

J N Demas

Author Affiliations +

Proceedings Volume 0910, Fluorescence Detection II; (1988) https://doi.org/10.1117/12.945456
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

Earlier we described two methods of reducing quenching effects in analytical luminescence measurements based on phase resolved spectroscopy (PRS). These methods are a form of time resolution based on the amplitudes and the phase shifts of modulated signals. In Method 1 the signal is modulated at a high frequency, and the ac signal is essentially independent of the degree of sample quenching. Method 2 simultaneously measures the modulated amplitude and the phase shift between the emission and the excitation; from these data one can correct the signal to that of the unquenched sample. A procedure is given for calculating the precision and accuracy of both methods for different noise levels and degrees of sample quenching. The merits of both methods are discussed. Method 1 is shown to have a lower noise level than Method 2, but suffers from systematic errors especially at low modulation frequencies. Method 2 is superior if lower modulation frequencies must be used and computational complexity and speed of response are less critical.

Citation Download Citation

J N Demas "Error Analysis Of Phase Resolution Methods For Elimination Of Quenching Effects In Luminescence Spectroscopy", Proc. SPIE 0910, Fluorescence Detection II, (27 April 1988); https://doi.org/10.1117/12.945456

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