CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10041 > Article
Presentation
19 April 2017 Endoscopic sensing of pH in the distal lung (Conference Presentation)
Debaditya Choudhury, Michael G. Tanner, Sarah McAughtrie, Fei Yu, Bethany Mills, Tushar R. Choudhary, Sohan Seth, Thomas Craven, James M. Stone, Ioulia K. Mati, Colin J. Campbell, Mark Bradley, Christopher K.I. Williams, Kevin Dhaliwal M.D., Timothy A. Birks, Robert R. Thomson
Author Affiliations +
Proceedings Volume 10041, Optical Techniques in Pulmonary Medicine II; 100410B (2017) https://doi.org/10.1117/12.2251925
Event: SPIE BiOS, 2017, San Francisco, California, United States
Abstract
In healthy humans, the physiological state in the distal lung alveolar acinar units is tightly regulated by normal homeostatic mechanisms. Pulmonary abnormalities such as chronic obstructive pulmonary disease, that are characterized by recurrent cycles of inflammation and infection involving dense infiltration by myeloid derived peripheral blood cells, may result in significant perturbation of the homeostatic baselines of physiology in addition to host tissue damage. Therefore, the ability to quantify and monitor physiology (e.g. pH, glucose level, oxygen tension) within the alveolar acinar units would provide a key biomarker of distal lung innate defence. Although in vitro modeling of fundamental biological processes show remarkable sensitivity to physiological aberrations, little is known about the physiological state of the distal lung due to the inability to concurrently access the alveolar sacs and perform real-time sensing. Here we report on previously unobtainable measurements of alveolar pH using a fiber-optic optrode and surface enhanced Raman spectroscopy (SERS) and show that alveolar pH changes in response to ventilation. The endoscope-deployable optrode consisted of para-mercaptobenzoic acid functionalized 150 nm gold nanoshells located at the distal end, and an asymmetric dual-core optical fiber designed for spatially separated optical pump delivery and SERS signal collection in order to circumvent the unwanted Raman signal originating from the fiber itself. We demonstrate a ~ 100-fold increase in SERS signal-to-fiber background ratio and pH sensing at multiple sites in the respiratory acinar units of a whole ex vivo ovine lung model with a measurement accuracy of ± 0.07 pH units.
Conference Presentation
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Debaditya Choudhury, Michael G. Tanner, Sarah McAughtrie, Fei Yu, Bethany Mills, Tushar R. Choudhary, Sohan Seth, Thomas Craven, James M. Stone, Ioulia K. Mati, Colin J. Campbell, Mark Bradley, Christopher K.I. Williams, Kevin Dhaliwal M.D., Timothy A. Birks, and Robert R. Thomson "Endoscopic sensing of pH in the distal lung (Conference Presentation)", Proc. SPIE 10041, Optical Techniques in Pulmonary Medicine II, 100410B (19 April 2017); https://doi.org/10.1117/12.2251925
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Lung
Surface enhanced Raman spectroscopy
Medical research
Endoscopy
Physiology
Biosensing
Blood
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top