Wide-field surface-enhanced CARS microscopy of cells (Conference Presentation)

Alexander Fast; John T. Kenison; Eric O. Potma

doi:10.1117/12.2254549

24 April 2017 Wide-field surface-enhanced CARS microscopy of cells (Conference Presentation)

Alexander Fast, John T. Kenison, Eric O. Potma

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 1006910 (2017) https://doi.org/10.1117/12.2254549
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We have previously demonstrated a total internal reflection, wide-field CARS microscope, where the signal is enhanced with the aid of a thin gold layer that supports surface plasmon polariton resonances. This surface-enhanced CARS microscope is capable of generating images of lipid structures in close proximity (<100 nm) to the glass substrate at excitation densities that are 4 orders of magnitude lower than in point-scanning CARS microscopy. In this contribution, we demonstrate its application to visualizing lipids in aqueous media, including imaging of cells, with a unique surface-sensitive contrast that cannot be obtained with conventional CARS microscopy.

Conference Presentation

Citation Download Citation

Alexander Fast, John T. Kenison, and Eric O. Potma "Wide-field surface-enhanced CARS microscopy of cells (Conference Presentation)", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006910 (24 April 2017); https://doi.org/10.1117/12.2254549

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