Irina Vasilenko,1,2 Vladislav Metelin,1,2 Aleksandr Kuznetsov,3 Vladimir Belyakov,3 Tatiana Yakushina2
1Maimonides State Classicial Academy (Russian Federation) 2M. F. Vladimirsky Moscow Regional Research and Clinical Institute (Russian Federation) 3Pirogov Russian National Research Medical Univ. (Russian Federation)
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
Quantitative phase imaging (QPI) can be considered as a potential tool to extract important information on the refractive index of the cellular and subcellular structures. Interphase chromatin is an original biosensor, a detector of early changes in morphofunctional cell condition. The authors presented a technology of densitometric segmentation based on the quantitative phase microscopy and computed analysis of the changes in the optic density of interphase chromatin as a biosensor. The purpose of this work to evaluate the possibility of quantitative phase imaging for visualizing the nuclear proteins conformation and chromatin decondensation degree. Modification of chromatin structure, compactness of its package, and so on, are indicative of cell condition alteration and may be projected on the organism as a whole not only for the early preclinical diagnostics but also for assessment of prognosis in crisis conditions.
Irina Vasilenko,Vladislav Metelin,Aleksandr Kuznetsov,Vladimir Belyakov, andTatiana Yakushina
"Opportunities of QPI in the epigenetic diagnostics and assessment of therapeutic efficacy", Proc. SPIE 10074, Quantitative Phase Imaging III, 1007417 (21 February 2017); https://doi.org/10.1117/12.2253033
ACCESS THE FULL ARTICLE
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Irina Vasilenko, Vladislav Metelin, Aleksandr Kuznetsov, Vladimir Belyakov, Tatiana Yakushina, "Opportunities of QPI in the epigenetic diagnostics and assessment of therapeutic efficacy," Proc. SPIE 10074, Quantitative Phase Imaging III, 1007417 (21 February 2017); https://doi.org/10.1117/12.2253033