The selection of light emitting diode irradiation parameters for stimulation of human mesenchymal stem cells proliferation

Rafał Lewandowski; Elżbieta A. Trafny; Małgorzata Stępińska; Andrzej Gietka; Paweł Kotowski; Monika Dobrzyńska; Mariusz P. Łapiński

doi:10.1117/12.2262445

8 December 2016 The selection of light emitting diode irradiation parameters for stimulation of human mesenchymal stem cells proliferation

Rafał Lewandowski, Elżbieta A. Trafny, Małgorzata Stępińska, Andrzej Gietka, Paweł Kotowski, Monika Dobrzyńska, Mariusz P. Łapiński

Author Affiliations +

Proceedings Volume 10159, Laser Technology 2016: Progress and Applications of Lasers; 101590E (2016) https://doi.org/10.1117/12.2262445
Event: XIth Symposium on Laser Technology, 2016, Jastarnia, Poland

Abstract

Human mesenchymal stem cells (hMSCs) with their vast differentiation potential are very useful for cell-based regenerative medicine. To achieve sufficient numbers of cells for tissue engineering, many different methods have been used to reach the effective increase of cell proliferation. Low-energy red light provided by light emitting diodes (LEDs) have been recently introduced as a method that promoted biomodulation and proliferation of hMSCs in vitro. The purpose of this study was to find the optimum stimulatory dosimetric parameters of LED (630 nm) irradiation on the hMSCs proliferation. The energy density was 2, 3, 4, 10, 20 J/cm² and the power density used was 7, 17 or 30 mW/cm². Human MSCs were irradiated with single or triple exposures daily at room temperature and the cell proliferation rate was evaluated during nine days after irradiation. The results showed that after irradiation 4 J/cm² and 17 mW/cm² at a single dose the proliferation rate of hMSCs increased on day 5 and 9 (13% and 7%, respectively) when compared to nonirradiated cells. However, triple LED irradiation under the same parameters resulted in the decline in the cell proliferation rate on day 5, but the proliferation rate was at the same level on day 9, when compared with the cell proliferation after irradiation with a single dose. The effect of a single dose irradiation with 4 J/cm² and 17 mW/cm² on the proliferation of cells was the highest when the cells were irradiated in phosphate-buffered saline (PBS) instead of MSCGM culture medium.

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Rafał Lewandowski, Elżbieta A. Trafny, Małgorzata Stępińska, Andrzej Gietka, Paweł Kotowski, Monika Dobrzyńska, and Mariusz P. Łapiński "The selection of light emitting diode irradiation parameters for stimulation of human mesenchymal stem cells proliferation ", Proc. SPIE 10159, Laser Technology 2016: Progress and Applications of Lasers, 101590E (8 December 2016); https://doi.org/10.1117/12.2262445

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