The study of radiation damage of yeast cells in Cryo-soft x-ray tomography

Liang Guo; Yong Guan; Chenxi Wei; Yue Hu; Yangchao Tian; Gang Liu

doi:10.1117/12.2268715

8 March 2017 The study of radiation damage of yeast cells in Cryo-soft x-ray tomography

Liang Guo, Yong Guan, Chenxi Wei, Yue Hu, Yangchao Tian, Gang Liu

Proceedings Volume 10255, Selected Papers of the Chinese Society for Optical Engineering Conferences held October and November 2016; 102551Q (2017) https://doi.org/10.1117/12.2268715
Event: Selected Papers of the Chinese Society for Optical Engineering Conferences held October and November 2016, 2016, Jinhua, Suzhou, Chengdu, Xi'an, Wuxi, China

Abstract

Cryo soft X-ray tomography (Cryo-SXT) is a valuable tool for high-resolution three dimensional (3D) imaging of cells in near-native preservation state. Cryo-immobilization of cells based on rapid cryogenic freezing is crucial to obtain good imaging in Cryo-SXT. In order to acquire high contrast image of live cells, it need to prepare the specimen ice as thin as possible. However, as the thickness of the ice layer is further reduced, the live cells may be destroyed during the tilt series data collection of 3D imaging due to radiation. Thus, it is essential to seek out a proper thickness range of the ice layer to get high contrast image without radiation damage of cells. For further understanding the relationship between thickness of ice layer and degree of radiation damage, yeast cells with different thickness of ice layer were imaged using Cryo-SXT. Finally the ice layer with thickness about 10 μm was confirmed to be proper for good imaging.

Citation Download Citation

Liang Guo, Yong Guan, Chenxi Wei, Yue Hu, Yangchao Tian, and Gang Liu "The study of radiation damage of yeast cells in Cryo-soft x-ray tomography", Proc. SPIE 10255, Selected Papers of the Chinese Society for Optical Engineering Conferences held October and November 2016, 102551Q (8 March 2017); https://doi.org/10.1117/12.2268715

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