Quantitative image analysis of angle scanning label-free surface plasmon resonance microscopy

Taehwang Son; Changhun Lee; Donghyun Kim

doi:10.1117/12.2289316

23 February 2018 Quantitative image analysis of angle scanning label-free surface plasmon resonance microscopy

Taehwang Son, Changhun Lee, Donghyun Kim

Proceedings Volume 10509, Plasmonics in Biology and Medicine XV; 1050906 (2018) https://doi.org/10.1117/12.2289316
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

In this report, we describe improvement of image resolution in surface plasmon resonance microscopy (SPRM) which suffers from poor quality due to severe surface plasmon (SP) propagation. Our approach takes two-channel momentum sampling by switched light incidence followed by minimum filtering to implement spatially switched SPRM (ssSPRM). The performance evaluated with periodic wires in comparison with conventional SPRM and bright-field microscopy shows that the effect of SP propagation can be circumvented and the effective decay length of SPRM is calculated to increase by only 7% compared to that of bright-field images.

Citation Download Citation

Taehwang Son, Changhun Lee, and Donghyun Kim "Quantitative image analysis of angle scanning label-free surface plasmon resonance microscopy", Proc. SPIE 10509, Plasmonics in Biology and Medicine XV, 1050906 (23 February 2018); https://doi.org/10.1117/12.2289316

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