CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10722 > Article
Presentation
17 September 2018 Using spider silk film to enhance biomolecular assembly and detecting efficiency in Bloch surface wave sensor (Conference Presentation)
Wei-Hung Chen, Kuan-Sheng Ho, Hsuan-Chen Wu, Yu-Ju Hung
Author Affiliations +
Proceedings Volume 10722, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVI; 107220W (2018) https://doi.org/10.1117/12.2321686
Event: SPIE Nanoscience + Engineering, 2018, San Diego, California, United States
Abstract
A surface plasmonic wave device for biological sensing is demonstrated. It consists of one-dimensional PMMA grating on an aluminum layer. This plasmonic device could increase the fluorescence intensity once the direction of the above dielectric grating and the pumping light could fit to the momentum matching condition. Here we incorporate a spider silk film into such fabricated plasmonic device as an intermediate layer to bridge the inorganic device surface and biological systems of interest. Specifically, a biocompatible spider silk film is casted on the device, followed by further grating fabrication and biomolecular conjugation via enzymatic reactions. Here in the research, antibodies and other proteins are assembled on the fabricated layer of spider silk film specifically with retained activities upon the enhanced fluorescence detection. Unlike other fabrication processes that require material-dependent surface treatments prior to biomolecular conjugation, the additional spider silk film provides an alternative strategy for facile and selective biological assembly in the biosensor. This, we envision, renders an unprecedented advantage for fabricating biosensors with various settings regardless of structural configurations or the building materials of the devices. Overall, the intermediate layer fabricated with spider silk under such grating structures not only demonstrates the strong surface field to enhance the pumping efficiency, but also enables binding of many target biomolecules for sensing and detection.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Wei-Hung Chen, Kuan-Sheng Ho, Hsuan-Chen Wu, and Yu-Ju Hung "Using spider silk film to enhance biomolecular assembly and detecting efficiency in Bloch surface wave sensor (Conference Presentation)", Proc. SPIE 10722, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVI, 107220W (17 September 2018); https://doi.org/10.1117/12.2321686
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Wave sensors
Plasmonics
Biosensing
Biosensors
Aluminum
Bridges
Dielectrics
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top