Vibrational imaging of metabolic dynamics in animals by DO-SRS (Conference Presentation)

Wei Min; Lingyan Shi

doi:10.1117/12.2509742

4 March 2019 Vibrational imaging of metabolic dynamics in animals by DO-SRS (Conference Presentation)

Wei Min, Lingyan Shi

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108821T (2019) https://doi.org/10.1117/12.2509742
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Metabolic dynamics is essential to unraveling the mechanistic basis of many biological processes, and cannot be imaged in vivo by using traditional methods. Here we developed a new method that combines D2O probing and SRS (DO-SRS) to visualize metabolic dynamics in live animals. The enzymatic incorporation of D2O-derived deuterium (D) into biomolecules will generate carbon-deuterium (C-D) bonds in macromolecules. We developed spectral unmixing methods to obtain C-D signals with macromolecular selectivity, and applied DO-SRS to reveal metabolic dynamics in C. elegans, zebrafish, and mice, to study developmental biology, aging, and tissue homeostasis, and to visualize tumor boundaries and metabolic heterogeneity.

Conference Presentation

Citation Download Citation

Wei Min and Lingyan Shi "Vibrational imaging of metabolic dynamics in animals by DO-SRS (Conference Presentation)", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108821T (4 March 2019); https://doi.org/10.1117/12.2509742

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