Identification of body fluids using metal enhanced fluorescence substrate with glancing angle deposited Ag nanorods (Conference Presentation)

Naseem Abbas; Xun Lu; Mohsin ali Badshah; Won il Heo; Sung Jun Seo; Seok-Min Kim

doi:10.1117/12.2511045

4 March 2019 Identification of body fluids using metal enhanced fluorescence substrate with glancing angle deposited Ag nanorods (Conference Presentation)

Naseem Abbas, Xun Lu, Mohsin ali Badshah, Won il Heo, Sung Jun Seo, Seok-Min Kim

Author Affiliations +

Proceedings Volume 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI; 108950F (2019) https://doi.org/10.1117/12.2511045
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

The identification of body fluids including blood, saliva, urine, sweat, semen, and vaginal fluid can be a vital evidence that can be used to identify a suspect and reconstructing the criminal case. Since the amount of evidence in the crime site is limited, a multiplex identification system for body fluid using a small amount of sample is prepared. In this research, we proposed a multiplex detection platform for semen and vaginal fluid, which were important for sexual crime using an Ag vertical nanorod metal enhanced fluorescence (Ag-MEF) substrate. The Ag-MEF substrate with a length of 500 nm was fabricated by glancing angle deposition and the Amin functionalization was conducted to improve the binding ability. The effect of incubation time was analyzed and an incubation time of 60 min was selected where the fluorescence signal was saturated. To examine the performance of the developed identification chip, an identification of the semen and virginal fluid was carried out. The developed sensor can selectively identify the semen and virginal fluid without any cross reaction. In addition, the limit of detection for semen was 10 times lower than that of the commercially available RSID-Semen kit.

Conference Presentation

Citation Download Citation

Naseem Abbas, Xun Lu, Mohsin ali Badshah, Won il Heo, Sung Jun Seo, and Seok-Min Kim "Identification of body fluids using metal enhanced fluorescence substrate with glancing angle deposited Ag nanorods (Conference Presentation)", Proc. SPIE 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI, 108950F (4 March 2019); https://doi.org/10.1117/12.2511045

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KEYWORDS

Luminescence

Metals

Nanorods

Silver

Blood

Sensors

System identification

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