Characterization of diffuse reflectance spectrum from vegetable leaves as the techniques of chlorophyll concentration measurement

Wilda Prihasty; Aulia M. T. Nasution; Sekartedjo Koentjoro; Iwan Cony Setiadi

doi:10.1117/12.2504888

11 April 2019 Characterization of diffuse reflectance spectrum from vegetable leaves as the techniques of chlorophyll concentration measurement

Wilda Prihasty, Aulia M. T. Nasution, Sekartedjo Koentjoro, Iwan Cony Setiadi

Author Affiliations +

Proceedings Volume 11044, Third International Seminar on Photonics, Optics, and Its Applications (ISPhOA 2018); 110440K (2019) https://doi.org/10.1117/12.2504888
Event: Third International Seminar on Photonics, Optics, and Its Applications (ISPhOA 2018), 2018, Surabaya, Indonesia

Abstract

Chlorophyll is a main biochemistry component for photosynthesis and a health indicator for plant. Chlorophyll concentration can be measured by non-destructive and non-contact method using Diffuse Reflectance Spectroscopy. Phantom consist of intralipid, aquades, gelatin and chlorophyll. Chlorophyll with certain concentration that known by absorption technique is used to be a primary stage for DRS. Non linear least square data fitting using diffuse reflectance mathematical model is used to determine parameters from phantom. The value of parameters is used for chlorophyll concentration measurement and will be compared with the chlorophyll concentration from absorption technique. When the system have been quantified well, the system will be used for chlorophyll concentration measurement for leaves without a complicated sample preparation such as absorption technique. Phantom reflectance spectroscopy has been tested and has a similar spectrum shape with leave's reflectance. Chlorophyll concentration measurement has been tested from three species of vegetables with three variation concentration and got error value for mustard, spinach and kale respectively 3%-10% ; 2.5%-10% and 16%-31%.

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Wilda Prihasty, Aulia M. T. Nasution, Sekartedjo Koentjoro, and Iwan Cony Setiadi "Characterization of diffuse reflectance spectrum from vegetable leaves as the techniques of chlorophyll concentration measurement", Proc. SPIE 11044, Third International Seminar on Photonics, Optics, and Its Applications (ISPhOA 2018), 110440K (11 April 2019); https://doi.org/10.1117/12.2504888

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KEYWORDS

Diffuse reflectance spectroscopy

Absorption

Data modeling

Reflectivity

Mathematical modeling

Absorbance

Fiber optics

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